lipL [2019-05-08 12:44:23]

GcvH

locus: BSU37640

pI: 5.27

mw: 31.27 kDa

protein length: 281 aa

gene length: 846 bp

function: lipoic acid metabolism

product: GcvH

essential: no

ec: 2.3.1.204

synonyms: ipa-90d,ywfL

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.7|Biosynthesis and scavenging of lipoic acid]

Gene

Coordinates: 3,863,415 → 3,864,260

The protein

Protein family

• Octanoyltransferase LipL family (single member, according to UniProt)

Structure

• [PDB|2P5I] (from ''Bacillus halodurans'', 56% identity)

Expression and Regulation

Operons

• genes: [gene|F2E881F4BE0968C75BD75A5EFF4C7CA642034796|lipL]
  description: [Pubmed|9353933]

Biological materials

Mutant

• MGNA-B667 (ywfL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1666 NBRP B. subtilis, Japan]
• BKE37640 (Δ[gene|F2E881F4BE0968C75BD75A5EFF4C7CA642034796|lipL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATGTAAAACCCTTTC, downstream forward: _UP4_TGAACGTTCTTCGCATGCCG
• BKK37640 (Δ[gene|F2E881F4BE0968C75BD75A5EFF4C7CA642034796|lipL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATGTAAAACCCTTTC, downstream forward: _UP4_TGAACGTTCTTCGCATGCCG

References

Reviews

• 27074917

Original publications

• 9353933,21338420,21338421